Surface chemistry modification of silica nanoparticles alters the activation of monocytes

MITAROTONDA R, , , , , , ; SARACENO M; TODONE M; GIORGI E; EMILIO L. MALCHIODI; DESIMONE MF; DE MARZI MC

Theraputic Delivery

Future Science

Lugar: Londres; Año: 2021 vol. 11 p. 443 - 459

2041-5990

Background: Nanoparticles (NPs) interaction with immune system is a growing topic of study.Materials & Methods: Bare and amine grafted silica NPs effects on monocytes/macrophagescells were analyzed by flow cytometry, MTT test and LIVE/DEAD® viability/cytotoxicityassay. Results: Bare silica NPs inhibited proliferation and induced monocyte/macrophagesactivation (increasing CD40/CD80 expression besides proinflammatory cytokines and nitritesecretion). Furthermore, silica NPs increased cell membrane damage and reduced the number ofliving cells. In contrast, amine grafted silica NPs did not alter these parameters. Conclusions:Cell activation properties of bare silica NPs could be hindered after grafting with amine moieties.This strategy is useful to tune the immune system stimulation by NPs or to design NPs suitableto transport therapeutic molecules.