INVESTIGADORES
MALCHIODI Emilio Luis
artículos
Título:
Modulatory effects of myocardial physiology induced by an anti-Trypanosoma cruzi monoclonal antibody involve recognition of major antigenic epitopes from 1-adrenergic and M2-muscarinic chlolinergic receptors without requiring receptor cross-linking.
Autor/es:
CREMASCHI G, FERNÁNDEZ MM, GORELIK G, GOING JC, FOSSATI CA, ZWIRNER NW & MALCHIODI EL.
Revista:
JOURNAL OF NEUROIMMUNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2004 vol. 153 p. 99 - 107
ISSN:
0165-5728
Resumen:
It has been proposed that anti-myocardial antibodies (Ab) against neurotransmitter (NT) receptors are involved in the immunopathology of chronic Chagas’ heart disease.  We demonstrated that an anti-T. cruzi monoclonal Ab (mAb), CAK20.12, binds to murine cardiac b-adrenergic and muscarinic acetyl choline (mACh) receptors eliciting abnormal physiological responses on normal heart.  No cross-linking requirement for mAb actions was demonstrated using Fab fragment derived from CAK20.12.  mAb binding to synthetic peptides from the second extracellular loop of both b1-adrenergic and mACh receptors, demonstrated by ELISA, identified the region of NT receptors involved.  Cross reactivity between these peptides and T. cruzi antigen was confirmed by binding inhibition assays.  These results support the existence of cross-reactivity due to molecular mimicry between a parasite antigen and the major antigenic epitopes present on both b1-adrenergic and M2-ACh receptors.  Its possible relationship with cardiac dysfunction during chronic stage of Chagas´ disease is also discussed