Identity of the major cysteine proteinase (cruzipain) from T. Cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody.

MALCHIODI EL, CHIARAMONTE MG, MARTINEZ JA, ZWIRNER NW, MARGNI RA, CAZZULO JJ.

IMMUNOLOGY LETTERS

Año: 1993 vol. 35 p. 59 - 62

0165-2478

Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony´s double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical.