Local Immunoglobulin E Production in Colorectal Polyps of Patients

CANZIANI, K.; PUCII MOLINERIS, M; BALCARCE, N; DÍAZ JIMENEZ D; GUZMAN, L; GARCIA, M; ALTAMIRANO E; MEIER, D; HERMOSO RAMELLO M; MUGLIA C.I.; DOCENA, G.

San Francisco

Congreso; Federation of Clinical Immunology Societies Annual Meeting; 2018

Federation of Clinical Immunology Societies

Juvenile polyps (JP) are common in children. The aetiology of JP remains largely unknown and few reports have characterized its stroma in atopic patients. We previously showed that 70 % of patients older than 1 yo, with rectal bleeding and JP had high levels of serum IgE specific to cow milk proteins (CMP). This finding prompted us to study the relation between the aetiology of polyps and the atopic condition of patients.Resected polyps (PT) and surrounding mucosa (SCT) were studied (n=10) by histopathology (H&E), confocal microscopy and RT-qPCR; cytokines were assessed by CBA and ELISA; germinal centers were isolated by dissection laser microscopy and analyzed by PCR. We found in 7 patients with serum CMP-specific IgE a cell infiltrate in the stroma of polyps dominated with eosinophils,mast cells, IgE-producing plasmatic cells , ST2+, TSLP+ and IL33+cells. SCT showed fewer frequencies of IgE+ cells (30±10 cells vs 2±1 polyps vs mucosa). Furthermore, we found higher transcription levels of il-4, il-5, il-33, ST2s (p+,Ki67+, AID+, and CD57+ (TFH). We demonstrated by PCR that IgE is produced through a direct and sequential class switch (μ to ε, μ to γ and to ε).In conclusion, we found in colorectal polyps an allergic inflammatory cell infiltrate with hyper eosinophilia and local production of IgE, which might be related to the atopic condition of patients.