INVESTIGADORES
MUGLIA Cecilia Isabel
congresos y reuniones científicas
Título:
Analysis of the eosinophil-chemoattractant cytokine production in intestinal tissues of patients with food allergy
Autor/es:
CANZIANI, K.; VACCARO, J.; ROLNY, I; ALTAMIRANO E; GUZMAN, L; BERNEDO, V; GONZALES VILLAR, MT; GARCIA, M; MUGLIA C.I.; DOCENA, G.
Lugar:
Mar del Plata
Reunión:
Congreso; SAI-SAIC-SAFIS 2018; 2018
Institución organizadora:
Sociedad Argentina de Inmunología, Sociedad Argentina de Investigación Clínica, Sociedad Argentina de Fisiología
Resumen:
Juvenile polyps (JP) are common in pediatric patients with milk allergy and lead to rectal bleeding and diarrhea. We have previously characterized the allergic inflammatory cell infiltrate of the polyp tissue, and here we aimed to investigate the origin of the high frequency of eosinophils, which may be responsible for tissue remodeling. Biological intestinal samples were obtained by colonoscopy in 7 patients, along with a biopsy of the surrounding tissue (SCT), as a control sample. The production of eotaxin-3 (CCL26) and RANTES, chemokines responsible for eosinophil homing, were assessed by qPCR and confocal microscopy in lamina propria and the epithelial compartment. In addition, Caco-2 and HMEC-1 cell lines were employed to study the induction of chemokine production. Cells were stimulated with IFN-γ (10ng/ml) and IL-13 (10ng/ml), and the activation of STAT-3 and expression of chemokines were analyzed by immunoblotting and qPCR, respectively. We found a significant increase of eosinophils and eotaxin-3 expression in polyps compared to SCT (p