Osmotic induction marking with Alizarin Red S on pejerrey, Odontesthes bonariensis juveniles

CAMPANELLA, D.; GARRIZ, A.; COLAUTTI, D. C.; SOMOZA, G.; MIRANDA, L. A.

NEOTROPICAL ICHTHYOLOGY

SOC BRASILEIRA ICTIOLOGIA

Lugar: Porto Alegre; Año: 2013 vol. 11 p. 95 - 100

1679-6225

Juveniles of pejerrey, Odontesthes bonariensis, were exposed to 0.1% Alizarin Red S (ARS) alone or with a previous immersion in 2.2% saline solution (Osmotic Induction, OI) to enhance the ARS marking method. The procedures were performed in the field, and fish were then released in 1 m3 cages in ?La Salada de Monasterio? lagoon, Chascomús, Buenos Aires, Argentina. After 73 days, clear marks were observed in the otoliths, caudal fin rays and scales with both treatments, being the intensity of the signal in the scales of OI+ARS treated fish higher. On the other hand, no marks were observed in the control group on the same structures. Approximately one year post-treatment (385 days), only marks in caudal fin rays were found clearly in OI+ARS treated fish. After this period, no significant differences in total length or weight between marked or control fish were observed and the mortality ranged between 30-40 % in all cages. These results provide strong evidence for the potential applicability of this cost-effective marking technique in differentiation of wild and hatchery-produced pejerrey, a much needed step in the advance of the O. bonariensis propagation and extensive aquaculture. The success in the caudal fin rays marking is also important because it is easy to do and does not require the sacrifice of fish.