CONTRATADOS
RETEGUI Lilia Alicia
congresos y reuniones científicas
Título:
CYTOKINES INVOLVED IN THE CHANGE OF ANTIBODY SPECIFICITY INDUCED BY LDV (LACTATE DESHYDROGENASE-ELEVATING VIRUS)
Autor/es:
MACARENA A. OTTOBRE SABORIDO, LILIA A. RETEGUI, JEAN-PAUL COUTELIER AND JOSÉ L. APARICIO1
Lugar:
Mar del Plata
Reunión:
Congreso; LXIV Reunión Anual de La Sociedad Argentina de Inmunología (SAI); 2016
Institución organizadora:
SAI
Resumen:
Introduction: LDV is a persistent and non pathogenic RNA arterivirus that induces NK, macrophages and B- cell activation in mice. It was found that LDV- infection modified Ab specificity to different antigens, depending on the genetic background of the host. Objectives: The purpose of this work was to study, through anti-cytokine auto-vaccination, the cytokines involved in the LDV effects. Methods: BALB/c, CBA/Ht and C57BL/6 mice were infected with 2×107 50% infectious doses of LDV in saline. Subsequently, the animals were inoculated in the footpads, four times at 2-wk intervals, with 2μg of IL-15/OVA, IL-23/OVA or IL-17/OVA complexes emulsified in Gerbu adjuvant. Ten days after the last inoculation the animals were bled. Serum lactate dehydrogenase (LD) was determined enzymatically, whereas IL-17 concentration was measured by ELISA. The proportion of Ab directed to native OVA epitopes was calculated by competition ELISA assays. Results: Vaccination with IL-15/OVA, IL-23/OVA or IL-17/OVA elicited similar titers of anti-OVA Ab in LDV-infected (1/160.000 to 1/300.000) and non-infected animals (1/145.000 to 1/300.000). The proportion of native anti-OVA Ab in BALB/c mice vaccinated with IL-15/OVA or IL-23/OVA was elevated and similar between non-infected (85 and 81%, respectively) and LDV-infected animals (80 and 84%). By contrast, control CBA/Ht mice vaccinated with IL-15/OVA or IL-17/OVA developed low titers of anti native OVA epitopes (56 and 38%, respectively) that increased to 81 and 71%, respectively, in LDV-infected animals. Furthermore, vaccination with IL-17/OVA augmented plasmatic IL-17 levels in LDV-infected animals in comparison with control (150±12 pg/ml and 23±4 pg/ml P
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