RETEGUI Lilia Alicia
congresos y reuniones científicas
-?Interleukin 17 (IL-17) is involved in some effects of Murine Hepatitis Virus (MHV-A59)?
JOSE L. APARICIO, MAITE DUHALDE VEGA, CATHERINE UYTTENHOVE, JACQUES VAN SNICK, JEAN-PAUL COUTELIER, LILIA A. RETEGUI
Congreso; First LASID-SAI-FAIC Meeting; 2015
Background: MHV-A59 triggers various mouse pathologies such as hepatitis, thymus involution and hypergammaglobulinaemia. We reported that viral infection induced autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH) and release of uric acid and high-mobility group box protein 1. Since IL-17 is a pro-inflammatory cytokine involved in autoimmune diseases, cancers and viral infections, we explored the effects of neutralizing IL-17 with a monoclonal antibody (MAb MM17F3) on MHV activity.Methods: C57BL/6 mice were infected with MHV-A59 (104 TCID50) and 4, 7 and 11days post-infection were inoculated with 150 ìg of anti IL-17 MAb (MM17F3). After infection, serum transaminases and uric acid concentration were determined enzymatically, immunoglobulins, IL-17 and IL-6 were measured by sandwich ELISA, and autoAb to FAH were detected by Western-blot. Liver histopathology was also explored.Results: Treatment of animals with the neutralizing MAb MM17F3 decreased the level of transaminases (P<0.05) as well as autoAb to FAH. By contrast, an increase of uric acid (P<0.05) and Ig concentration in MHV-infected animals were detected (P<0.001). MM17F3 also increased the plasmatic IL-17 half-life as well as IL-6 concentration in infected and non- infected mice (9.4±0.7 and 11.2±0.6 pg/ml, respectively). Furthermore, livers from control animals showed a very mild degree of fibrosis whereas MHV- infected mice, treated or not with the MAb, presented moderate to severe degree of fibrosis.