SOLID SILICA-FUNCTIONALIZED MAGNETICNANOPARTICLES AS DRUG TARGETING DEVICES:DRUG INCORPORATION AND ENDOTHELIAL CYTOTOXICITY.

MARIELA AGOTEGARAY; ADRIÁN CAMPELO; VIRGINIA MASSHEIMER; VERÓNICA LASSALLE

Mar del Plata

Encuentro; VII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE NANOMEDICINA; 2016

IntroductionSolid silica(Si) coated magnetic nanoparticles(MNPs) are potential devices for drug targeting. Stability and biocompatibility of silica turn these systems feasible for biomedical applications although drug loading is a challenge due to silica inertia. ObjectiveSynthesis, characterization and Diclofenac(Dic) incorporation onto citric acid(CA)-functionalized magnetite(MG) coated with Si and 3-aminopropyltriethoxysilane(APTES). Evaluation of cytotoxicity on rat aortic endothelial cells(EC). Design MG/CA NPs were obtained by co-precipitation. Si and APTES functionalization was performed by a modified Stöber process, prolonged for 12h at r.t, using ratios MG/CA:TEOS:APTES=(1:0.5:2; 1:1:2; 1:2:2), named 1, 2, and 3. They were characterized by FTIR, DLS, z potential and HR-TEM microscopy. Dic was bonded by N,N´-dicyclohexylcarbodiimide and studied by UV-Vis spectroscopy. Primary cultures of EC were exposed for 48h to final concentrations of 1, 10 and 100µg/mL of MNPs and Dic-loaded MNPs. Cell viability was studied by MTT assay and by the capacity to produce NO(DAN assay).Results1 and 2 presented Dh of 400.0±10.0nm and 520±10.0nm; z of 27.9±5.78mV and 21.9±6.02mV in aqueous dispersions. 3 was polydisperse with z 32.6±5.81mV. HR-TEM micrographs showed matrix dispersed structures for the MNPs. Formulation 2 incorporated approx. 40% of Dic. Cell viability was not affected at 10µg/mL or below for all the samples (p