Biocompatible Magnetic Nanoparticles based on iron oxide and Arabic gum with potential in the treatment of inflammatory deseases

PAMELA AZCONA; MARÍA FERNANDA HORST; MARIELA AGOTEGARAY; VERÓNICA LASSALLE

Congreso; 8vo Congreso Latinoamericano de Organos artificiales, Biomateriales e Ingeniería de Tejidos; 2014

BIOCOMPATIBLE MAGNETIC NANOPARTICLES BASED ON IRON OXIDE AND ARABIC GUM WITH POTENTIAL IN THE TREATMENT OF INFLAMMATORY DESEASES Azcona Pamela, Horst Fernanda, Agotegaray Mariela, Lassalle Verónica INQUISUR-Dpto. de Química, Universidad Nacional del Sur, Bahía Blanca, Buenos Aires, Argentina Magnetic nanoparticles (MGNPs) are used for drug delivery, diagnostic-magnetic resonance imagining, etc. Factors such as size, surface area, and magnetic properties are very important for their application in the mentioned areas. The tendency to agglomerate for nanoparticles in suspension complicates the processing of stable nanoparticles suspensions and nanocomposites with a high degree of particles dispersion. There are many surface modification agents able to improve the colloidal stability of nanoparticles through different mechanisms but the chemistry of some of dispersants makes them unsuitable for use in biomedical applications. In this context, Arabic gum (AG) has emerged as an attractive option as modifier and/or stabilizer of magnetic phase. AG consists of three fractions with distinct chemical structures of polysaccharides; it is biocompatible and provides enough functional groups able to interact with magnetic core as well as drugs or other molecules of biological interest. The goal of this study is to prepare MNPs coated with AG though a simple, non-time consuming and cheap method. Two synthetic pathways have been explored to incorporate the AG to the magnetic core: i- GA coated MNPs, employing the biopolymer in solid state and ii-using a solution of AG. FTIR analysis show the presence of characteristics bands of GA and Fe3O4 confirming the incorporation of biopolymer. The hydrodynamic average size was measured in aqueous dispersions of the MMGNPs-AG obtaining values in the range of 50-80 nm. These sizes were consistent with the ones obtained from TEM images using the same dispersions. Magnetic measurements reveal a satisfactory magnetic response and allow an estimation of the MGNPs-AG composition that was in agree with the results arising from inductive coupling plasma (ICP) technique. It was found iron oxide contents of about 43 and 54% depending on the synthetic method. The potential of these nanocarriers was evaluated in terms of their ability to bind to a model non-steroidal antinflammatory such as Diclofenac. For this purpose 50 mg of carrier were dispersed in 100 mL of water and 100 mg of sodium diclofenac were incorporated. The dispersion was maintained under stirring at r.t during 24 hs. The evolution of MGNPs-AG properties in presence of the drug has been analyzed by measuring the Z potential and the average hydrodynamic diameter. An estimation of the efficiency of loading has been achieved by thermogravimetric analysis as well as by UV/visible spectroscopy. In the Figure below water dispersions of MGNPs-AG are depicted. Image of an aqueous dispersion of MGNPs-AG.