Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles

MARIELA AGOTEGARAY; V. LASSALLE

International journal of chemical and pharmaceutical analysis

Aarya Publishing House.

Año: 2014 vol. 1 p. 154 - 164

2348-0726

ABSTRACTMagnetic nanoparticles (MNPs) composed of magnetite coated with oleic acid and functionalized with chitosan have been prepared to thetargeted release of the non steroidal anti-inflammatory drug (NSAID) Diclofenac. Two procedures to incorporate the drug were explored:simple adsorption and covalent linkage. The impact of experimental variables such as the nature of the NSAID (commercial versusanalytical sodium Diclofenac), its concentration and amount of coupling agent were evaluated in terms of the loading efficiency and theproperties of interest of MNPs (size, surface functionality, stability). Loaded and unloaded MNPs were characterized by FTIR-DRIFTSspectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. UV-visible quantification of Diclofenac wasachieved reaching values of loading efficiency between 11 and 27% depending on the experimental conditions. Probable mechanisms fordrug-MNPs interactions have been proposed on the base of characterization data.A nanocarrier of size lower than 150 nm, with satisfactory loading skill and high dispersion capability on water was obtained. The releasebehavior demonstrated to be independent on the presence of an external magnetic field. The properties found in these nanosystemsresult relevant in view of their effective in vivo implementation.