INVESTIGADORES
LANUSSE Carlos Edmundo
artículos
Título:
Efficacy of oxyclozanide against adult Paramphistomumleydeni in naturally infected sheep.
Autor/es:
SANABRIA, R; MORENO, L; ALVAREZ, L; LANUSSE, C; ROMERO, J
Revista:
VETERINARY PARASITOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 206 p. 277 - 281
ISSN:
0304-4017
Resumen:
tThe aim of the current study was to assess oxyclozanide (OCZ) efficacy against Paramphis-tomum leydeni in naturally infected adult sheep. OCZ concentrations in blood stream andgastrointestinal fluids collected from treated animals were also measured. Fifteen P. leydeninaturally infected sheep were randomly divided into two groups: untreated control (n = 5)and treated (n = 10). The treated group was orally drenched with OCZ (20 mg/kg, day 0). Asecond dose was administered 72 h later. Faecal samples were taken at days 0, +3 and +5.Five sheep from both groups were slaughtered at day +5. At necropsies, rumen, abomasumand small intestine were examined for adult and immature flukes. All recovered flukes werecounted and the treatment efficacy was estimated. Additionally, serum and gastrointestinalfluid content (ruminal, abomasal and small intestine) samples, obtained from five treatedanimals at day +5, were analyzed by HPLC to measure OCZ concentrations. OCZ showedhigh efficacy (99%) against mature P. leydeni. The post-treatment egg reduction was alsohigh after the first dose with values ranging from 98.4% (day +3) to 99.5% (day +5). Thehighest OCZ concentrations were measured in serum (20.7 ± 11.5 g/mL) followed by thesmall intestinal fluid (6.00 ± 4.50 g/mL). Very low OCZ concentrations (ranging between0.05 and 0.02 g/mL) were measured in ruminal and abomasal fluids. OCZ administered tosheep twice (20 mg/kg) by the oral route was highly efficacious against mature stages of P.leydeni in naturally infected sheep. Despite a high drug concentration at the intestinal fluid,OCZ efficacy against immature stages could not be assessed. OCZ efficacy and assessmentof its concentration profiles in different tissues are considered a contribution to the scarceinformation available on this ruminant fluke.
