Calbindin distribution in dentate gyrus layers of patients with depression and Resistant temporal lobe epilepsy

DALESSIO L ; KONOPKA H; ACUÑA A; ESCOBAR E; SCÉVOLA L; FERNANDEZ LIMA M; SEOANE E; KOCHEN S

Estambul

Congreso; 31th International Epilepsy Congress; 2015

Purpouse: The aim of this study was to determine the immunoreactivity for calbindin (expressed in post-mitotic new generated cells during sinaptogenesis), in dentate gyrus layers of patients with resistant TLE (temporal lobe epilepsy) and hippocampal sclerosis (HS) with and without depressive disorders. Both depression and TLE have been linked to abnormalities in hippocampal neuroplasticity but pathogenic mechanisms are still unclear. Methods:Patients with HS and TLE were evaluated according clinical, neurological and psychiatric assessment protocols before surgery. For psychiatric diagnoses, Structured Clinical Interviews for DSM IV Disorders (SCID-I and ?II) were determined.After surgery selected hippocampal sections from patients with and without depressive disorders and archival material from normal post-mortem hippocampus were processed using immunoperoxidase with antibody anti-calbindin. Reactive area for calbindin, number of positive cells per field (20X), and MGV (mean gray value) were measured by computerized image analysis (Image J). ANOVA Test was determined.Results:Hippocampal sections of 8 patients with TLE and depression (TLE+D), 8 patients with TLE without depression, and 5 matched postmortem controls were included. Calbindin immunoreactivity (Calbindin IR) was found in granule cells layers of both controls and patients. A significant reduction in the total number of calbindin IR cells per field was found in patients, more intense in TLE +D