INVESTIGADORES
BARBEITO ANDRES jimena
congresos y reuniones científicas
Título:
Brain cell composition analysis through the isotropic fractionator method in a murine experiment of maternal malnutrition
Autor/es:
BARBEITO - ANDRÉS J, CASTRO E, BERNAL V, HALLGRIMSSON B, LENT R, GONZALEZ PN
Reunión:
Congreso; 2nd Congress of the Federation of Latin American and Caribbean Neuroscience Societies; 2016
Resumen:
Nutrition isone of the environmental factors that mostly affects brain development.Different behavioral, cognitive, physiological and anatomical impairments areassociated with nutritional restriction during early life. However, it is stilllargely unknown if changes in quantitative cellular composition (number, celltype proportions) in different brain regions occur as a result of maternalmalnutrition. Using a mouse experimental model of maternal protein and caloricundernutrition, we assessed absolute cell number in six brain regions of theoffspring by means of the isotropic fractionator method. This method consistson breaking the cell membranes and releasing the nuclei into a fluid of knownvolume. From this isotropic solution, aliquots are extracted and the density ofcells is determined. All nuclei were stained and counted by means of DAPI whilethe proportion of neurons was determined using NeuN. Results indicated asignificant reduction of the total number of cells in the cerebellum and thecortex under protein restriction. Changes in number of neurons varied acrossregions. In sum, this study enlarges our knowledge on brain susceptibility todisrupting factors during brain development.