Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray

JUAN IGNACIO FERNANDINO; POPESKU, JP; PAUL-PRASANTH, B; MATSUDA, M; XIONG, H; HATTORI, R.S.; CARLOS AUGUSTO STRÜSSMANN; GUSTAVO MANUEL SOMOZA; NAGAHAMA, Y; TRUDEAU, VL

Buenos Aires

Jornada; XI Jornadas de la Sociedad Argentina de Biología; 2009

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:FR;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ascii-font-family:Calibri; mso-fareast-font-family:Calibri; mso-hansi-font-family:Calibri;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> Gonadogenesis is the process related with the morphological development of a differentiated. Studies on gonadal gene expression with sex-related dimorphism are important to increase our understanding on gonadogenesis and to investigate how environmental factors such as temperature can regulate or even direct gonadal development. Was used an EST microarray slide obtained from medaka (Oryzias latipes) larvae gonads, reared at male- and female-producing temperatures (MPT and FPT respectively) in the gonadal differentiation period. A total of 94 different transcripts were identified to be induced at MPT, whereas only 30 were found at FPT. Three differentially expressed genes were selected for microarray validation and analyzed by RQPCR and in situ hybridization. The cross-species hybridization correctly identified several robust expression genes, providing a powerful tool to further studies in this specie. Particularly, we focus on the interplay of apoptotic genes in relation to the masculinization.