INVESTIGADORES
FERNANDINO Juan Ignacio
artículos
Título:
Extrahypophyseal expression of gonadotropin subunits in pejerrey Odontesthes bonariensis and effects of high water temperatures on their expression.
Autor/es:
ELISIO, M.; SORIA, F.; JUAN IGNACIO FERNANDINO; CARLOS AUGUSTO STRÜSSMANN; GUSTAVO MANUEL SOMOZA; LEANDRO ANDRES MIRANDA
Revista:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2012 vol. 175 p. 329 - 336
ISSN:
0016-6480
Resumen:
It has been traditionally accepted that gonadotropins (GtHs), follicle stimulating hormone (FSH) and luteinizing hormone (LH), are synthesized and secreted only by the pituitary. However, in mammals, and recently in different fish species, it has been demonstrated the presence of theses hormones in extrapituitary tissues. In this study, as a first step fragments of FSH-β, LH-β and the glycoprotein hormone α (GPH-α) subunits cDNAs have been characterized in brain and gonadal tissue of pejerrey of both sexes at different stages of maturation. The in situ hybridization studies revealed that, in addition to their classical location in pituitary cells, the three GtHs transcripts were also located in the gonads but not in the brain. The presence of FSH-β and GPH-α subunits was found in the cytoplasm of oogonia, previtelogenic and vitellogenic oocytes in ovaries during vitellogenesis. A similar distribution with the exception of oogonia was observed for LH-β expression. In males, these three subunits were seen to be expressed in spermatogonia and spermatocytes. In spite of the function of extraphypophyseal GtHs is not completely understood, the exposure of fish to high water temperatures that impair pejerrey reproduction, also induced an decrease of extrapituitary GtHs subunit expression.