INVESTIGADORES
MAIALE Santiago Javier
congresos y reuniones científicas
Título:
Characterization of photosystem parameters of transplastomic lines expressing vaccine antigens
Autor/es:
ROMINA ALBARRACIN; MARIANA CORIGLIANO; SANTIAGO MAIALE; MARINA CLEMENTE
Reunión:
Congreso; 1 Annual Meeting. Argentine Society for Biochemistry and Molecular Biology Research; 2015
Resumen:
Chloroplaststransformation technology has emerged as an alternative platform for antigenexpression in plants. In particular, we demonstrated that the fusion of Toxoplasma gondii SAG1 antigen to Leishmania infantum Hsp83 (LiHsp83-SAG1)significantly increased the level of SAG1 accumulation in chloroplasts comparedto SAG1 alone. However, LiHsp83-SAG1 line showed chlorotic phenotype and growthretardation. In order to understand these unintended pleiotropic effects, we characterizedthe photosystem parameters of LiHsp83-SAG1 and compared them with SAG1 and GRA4transplastomic lines versus wild type plants (WT). Completely developed leavesfrom 42 old-days plants were used to measure foliar area, chlorophyll contentby chemical and spectrophotometry (SPAD) methods and chlorophyll fluorescence(OJIP). SAG1 foliar area was 17% bigger than WT, while LiHsp83-SAG1 was 77%smaller than WT. SPAD and chemical methods showed similar results,transplastomic lines had less chlorophyll content than WT. OJIP analysis showedno differences among SAG1, GRA4 and WT plants. On the other hand, LiHsp83-SAG1revealed low values for most of the parameters measured, such as maximumquantum yield of primary photosystem II photochemistry and electron transportper excited cross section. This first approach suggests that photosystemparameters in LiHsp83-SAG1, especially those related to PSII, are damaged.