INVESTIGADORES
MAIALE Santiago Javier
congresos y reuniones científicas
Título:
Polyamines and saline stress in plants
Autor/es:
SANCHEZ DIEGO; CUEVAS JUAN C; CHIESSA AMALIA; MAIALE SANTIAGO; GUIRADO ALEJANDRA; SANNAZARO ANALIA; PIECKENSTAIN FERNANDO; RUIZ OSCAR
Lugar:
villa Carlos Paz, Cordoba
Reunión:
Congreso; 28 Reunion anual de la sociedad argentina de investigaciones en bioquimica y biologia molecular; 2002
Institución organizadora:
Sociedad Argentina en Investigaciones en Bioquimica y Biologia Molecular
Resumen:
Polyamines are ubiquitous cellular components to play an essential role in growth and development. In plants, these amines are important in many aspects of development but their exact role needs to be established. Intracellular levels of polyamines are related to many developmental events such as cell division, flower formation, membrane stability, pathogen stress protection, as well as senescence. Recently, with the cloning of the genes for some polyamine biosynthetic enzymes, a number of studies have involved their artificial overexpression in plant system. In our lab we evaluated the changes in polyamine biosynthetic enzymes and polyamine levels in Lotus sp., rice and oat, under short and long saline stresses. On the base of these results we generated Lotus transgenic plants overexpressing the spermidine synthase obtained from tobacco under control of 35S promoter and other lines transformed with arginine decarboxylase (ADC) but under the control of rd29A, an inducible promoter cloned from Arabidopsis thaliana by genomic PCR. Previously we analyzed rd29A promoter expression in hairy root transformed with ADC and GUS, observing a temporal-biphasic induction by cold and salt exposition. He physiological relevance of spermine accumulation observed in plants under saline stress in the regulation of polyamine (PAO) and NADPH oxidase activities will be discussed.