INVESTIGADORES
MAIALE Santiago Javier
congresos y reuniones científicas
Título:
Transgenic Lotus corniculatus plants expressing spermidine synthase from tobacco
Autor/es:
SANNAZZARO ANALÍA; SÁNCHEZ DIEGO; CUEVAS JUAN C; MAIALE SANTIAGO; ALTABELLA TERESA; TIBURCIO ANTONIO; PIECKENSTAIN FERNANDO; RUIZ OSCAR
Lugar:
villa Carlos Paz, Cordoba
Reunión:
Congreso; 27 Reunion anual de la Sociedad Argentina de Investigaciones en bioquimica y biología molecular; 2001
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquimica y Biología Molecular
Resumen:
Lotus corniculatus is an agronomically important forage legume. Genetic engineering offers opportunities both to improve L.corniculatus as a crop and to increase basic understanding of plant biochemistry and metabolism. We have produced and analyzed transgenic plants harboring the spermidine synthase gene from Nicotiana tabacum expressed under the production of spermidine, a polyamine which is associated with morphogenesis, development and stress responses. A number of transgenic lines were obtained (as confirmed by PCR) and some of them were chosen for further analysis. We have determined their polyamine content by HPLC and spermidine synthase activity by radiochemical methods. We are also studying the expression of the transgene and the NADPH oxidase activity of the transgenic lines. Spermidine synthase activity varied among transgenic lines. The activity of some lines was similar to control (non-transformed) plants, whereas other lines exhibited higher values. Both spermidine and spermine levels were significantly higher in those lines showing high spermidine synthase activity, as compared with controls. It has been reported that tolerant plants from other species accumulate these two polyamines under saline stress conditions. Thus, we are currently evaluating salt tolerance of the transgenic lines obtained in this study. Lotus corniculatus is an agronomically important forage legume. Genetic engineering offers opportunities both to improve L.corniculatus as a crop and to increase basic understanding of plant biochemistry and metabolism. We have produced and analyzed transgenic plants harboring the spermidine synthase gene from Nicotiana tabacum expressed under the production of spermidine, a polyamine which is associated with morphogenesis, development and stress responses. A number of transgenic lines were obtained (as confirmed by PCR) and some of them were chosen for further analysis. We have determined their polyamine content by HPLC and spermidine synthase activity by radiochemical methods. We are also studying the expression of the transgene and the NADPH oxidase activity of the transgenic lines. Spermidine synthase activity varied among transgenic lines. The activity of some lines was similar to control (non-transformed) plants, whereas other lines exhibited higher values. Both spermidine and spermine levels were significantly higher in those lines showing high spermidine synthase activity, as compared with controls. It has been reported that tolerant plants from other species accumulate these two polyamines under saline stress conditions. Thus, we are currently evaluating salt tolerance of the transgenic lines obtained in this study.