INVESTIGADORES
GARRIZ Andres
congresos y reuniones científicas
Título:
Transcription Reprogramming in Lotus japonicus plants under foliar infection by Pseudomonas syringae
Autor/es:
BORDENAVE C; SANCHEZ DH; CARRASCO P; SERNA E; ESCARAY F; RUIZ OA; GARRIZ A
Lugar:
Pto Madryn
Reunión:
Congreso; Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2010
Institución organizadora:
Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Resumen:
Legume plants have the ability to establish symbiotic interactionswith rhizobia bacteria and mycorrhizal fungi, a trait that make themable to growth in poor soils. It is known that, at least partially, thedevelopment of symbiosis is regulated by plant defense genes thatare equally activated by natural pathogens. Therefore, theidentification of these genes is an interesting technological aim.Unfortunately, the molecular mechanisms that participate in thedefense of legume plants to pathogens remain still unclear. To addmore light to this field, the main aim of this work was tocharacterize the transcriptomic changes that occur in both,susceptible and resistant ecotypes of the model legume L. japonicusupon the infection caused by P. syringae. We present here anoptimized infection protocol and our first results of an AffymetrixGene chip analysis on a susceptible cultivar. Our experimentsshowed a notable transcription reprogramming in infected leaves,where most of the down-regulated genes correspond to thoserelated to photosynthesis and starch metabolism. On the other hand,in the up-regulated group those responsible for flavonoidbiosynthesis are predominant (mostly chalcones andanthocyanins). In addition, we identified several transcriptionfactors that could play an important role in plant defense. Thesimilarities with previously published reports are discussed.