INVESTIGADORES
CORVI Maria Martha
congresos y reuniones científicas
Título:
Impact of protein fatty acylation in Toxoplasma gondii
Autor/es:
ALONSO, AM; CABALLERO, MC; CORVI, MM
Lugar:
Mar del Plata
Reunión:
Congreso; LI Annual Meeting- Argentine Society for Biochemistry and Molecular Biology Research (SAIB); 2015
Institución organizadora:
SAIB
Resumen:
In higher eukaryotes protein fattyacylation plays key roles in cellular signaling pathways, in mediatingsubcellular targeting and in protein-protein interactions required foractivity. However little is known of this modification in apicomplexan parasites.With the aim of unveiling the role of protein fatty acylation in T. gondii, we analyzed the impact ofprotein palmitoylation and myristoylation in this parasite. The use of thebiotin-exchange method allowed us to obtain the T. gondii palmitoylome, which shows that these proteins are found in multiple cellular compartments, with a widerange of functions that vary from metabolic processes, gliding and host-cellinvasion to even regulation of transcription and translation. Furthermore,it is evident that this modification affects the majority of cellular functionssince we were able to identify proteins involved in signaling, glidingmotility, transcription and translation. In parallel, anin silico analysis shows that T. gondii expresses one N-myristoyltransferase and that myristoylated proteins are predicted to be involved inimportant events for this parasite such as calcium homeostasis and kinaseactivity regulation which are critical for invasion and gliding. Therefore, ourdata indicates that protein fatty acylation is a general mechanism of proteinregulation that affects vital processes of this parasite.