Metalloproteomics analysis in human mammary cell lines treated with inorganic mercury

MARIÁNGELES ÁVILA MANIERO; PATRICIA SMICHOWSKI; EDUARDO CALLEGARI; RODOLFO WUILLOUD; MARIEL FANELLI

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

ELSEVIER GMBH

Año: 2020 vol. 58 p. 126441 - 126453

0946-672X

The interest in inorganic Hg toxicity and carcinogenicity has been pointed to target organs such as kidney, brainor placenta, but only a few studies have focused on the mammary gland. In this work, analytical combinationtechniques (SDS-PAGE followed by CV-AFS, and nanoUPLC-ESI-MS/MS) were used to determine proteins thatcould bind Hg in three human mammary cell lines. Two of them were tumorigenic (MCF-7 and MDA-MB-231)and the other one was the non-tumorigenic cell line (MCF-10A). There are no studies that provide this kind ofinformation in breast cell lines with IHg treatment. Previously, we described the viability, uptake and the subcellular distribution of Hg in human breast cells and analysis of RNA-seq about the genes that encode proteinswhich are related to cytotoxicity of Hg. This work provides important protein candidates for further studies of Hgtoxicity in the mammary gland, thus expanding our understanding of how environmental contaminants mightaffect tumor progression and contribute with future therapeutic methods