An l-Lactate Amperometric Enzyme Electrode Based on l-Lactate Oxidase Immobilized in a Laponite Gel

VERONICA PAZ ZANINIE MISHA; BEATRIZ LÓPEZ DE MISHIMA; PIERRE LABBÉ; VELIA SOLÍS*

ELECTROANALYSIS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2010 vol. 22 p. 946 - 954

1040-0397

Abstract A biosensor based on the immobilization of Lactate oxidase in laponite – organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.330.01) A cm2 M1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. very high sensitivity (0.330.01) A cm2 M1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.330.01) A cm2 M1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. 2 M1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods. in good agreement with those of two standard methods. l-lactate determinations were in good agreement with those of two standard methods.