Probiotic potential of Lactobacillus delbrueckii strains and their phage resistant mutants

GUGLIELMOTTI, D. M.; BRIGGILER MARCÓ, M.; GOLOWZCYC, M.; REINHEIMER, J.; QUIBERONI, A. DEL L.

INTERNATIONAL DAIRY JOURNAL

Elsevier

Año: 2007 p. 916 - 925

0958-6946

Three commercial phage sensitive strains of Lactobacillus delbrueckii and spontaneous phage resistant mutants isolated from them were studied by focusing on their biological and probiotic features. After incubation in pH 2.0 simulated gastric solution, cell viable counts decreased moderately, but a protective effect on strains was observed when milk was added. A scarce resistance to bile was observed, while most of the strains tolerated lysozyme, grew in the presence of bile salts and fermented prebiotics. Some strains showed high hydrophobicity values and b-galactosidase activity. The strong antibacterial activity displayed toward pathogens was due to the production of lactic acid.  Sensitive strains and their phage resistant variants were able to adhere to Caco-2/TC-7 monolayers and significantly inhibited the invasion of Salmonella enterica serovar Enteritidis into Caco-2/TC-7 cells. All strains tested showed interesting potential probiotic features, indicating the usefulness of a reconsideration of the probiotic role of Lactobacillus delbrueckii.