INVESTIGADORES
ZARATE Sandra Cristina
congresos y reuniones científicas
Título:
In search of the serotonin role in the contrasting synapse remodeling induced by fluoxetine in cortical and hippocampal neurons
Autor/es:
MALLEVILLE CORPA MARÍA JOSÉ; TRAETTA MARIANELA; CODAGNONE MARTIN; UCCELLI NONTHUE; LITVAK EINAV; ZÁRATE SANDRA; REINÉS ANALÍA
Reunión:
Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018
Resumen:
The antidepressant fluoxetine (FLX) is a specific serotonin (5HT) reuptake inhibitor (SSRI). We have previously showed that behavioral benefits induced by FLX in experimental depression occur concomitantly with hippocampal changes in synapse morphology and number. FLX has also shown to increase synapse number in the cerebral cortex of naïve animals, an effect not shared by all SSRIs. The aim of this work was to study the in vitro profile of FLX-induced synapse remodeling in hippocampal and cortical neurons. To this aim, primary neuronal cultures obtained from embryonic (E18) and postnatal (P1-2) rats were exposed for 24 hours to FLX or 5HT. Immunostaining of the dendritic marker MAP-2 and the synaptic marker synaptophysin (SYN) were evaluated to study dendritic and synapse remodeling, respectively. In cortical neurons (DIV7), FLX treatment (1µM) increased SYN puncta number and total puncta area without modifying the dentritic tree. This effect was mimicked by 5HT and blocked by ketanserin (5HT2R antagonist). In hippocampal neurons (DIV14), FLX treatment (0.1-1 µM) decreased SYN puncta number and total puncta areaand induced dendritic retraction. 5HT treatment failed to mimic FLX effect in hippocampal neurons. Our results indicate that FLX-induced synapse remodeling depends on the neuronal phenotype and suggest thatwhile FLX effect in cortical neurons is 5HT-mediated, it seems to involve a more complex mechanism in hippocampal neurons.