PERSONAL DE APOYO
MARIN VIEGAS Vanesa Soledad
Vacuolar deposition of recombinant proteins in plant vegetative organs as a strategy to increase yields
VANESA SOLEDAD MARIN VIEGAS; CAROLINA GABRIELA OCAMPO; SILVANA PETRUCCELLI
Delivery of recombinant proteins to vegetative tissue vacuoles was considered not convenient since this compartment was expected to be hydrolytic. Nevertheless, as a consequence of environmental changes, development and even ectopic deposition of proteins, vacuole properties can be modified. Vacuolar sorting in vegetative tissues has been used as a strategy to enhance foreign protein yields, and this commentary analyzes selected examples that assess this approach. Stable vacuolar accumulation of toxic proteins such as avidin, cellulolytic enzymes, endolysin, and transglutaminase was proven to be an efficient strategy to increase yields and reduce the detrimental effect on plant growth. Also human collagen, glucocerebrosidase, α1-proteinase inhibitor, complement-5a, interleukin-6 and immunoglobulins were stable and accumulated at high yields in leaves vacuoles and suspension culture cells. To reach this final destination, fusions to sequence specific vacuolar sorting signals(ssVSS) typical of hydrolases and/or Ct-VSS classic of storage proteins were used and both types of motifs were capable to increase accumulation. The obtained results were dependent on the nature of foreign protein and also the physiological state of plants. The N-glycosylation patterns of vacuolar sorted proteins were variable, which indicates that vacuolar trimming of glycans does not necessarily occur. This fact also suggests that foreign proteins can reach the vacuole by two different pathways: direct transport from the ER, bypassing the Golgi or trafficking through the Golgi.