INVESTIGADORES
LAVARIAS Sabrina Maria Luisa
congresos y reuniones científicas
Título:
DETOXIFICATION SYSTEM INDUCTION, ANTIOXIDANT RESPONSE AND OXIDATIVE STRESS LEVELS IN THE ENTOMOPATHOGENIC FUNGUS Beauveria bassiana EXPOSED TO THE PYRETHROID DELTAMETHRIN.?
Autor/es:
FORLANI, LUCAS; JUÁREZ, PATRICIA; LAVARIAS, SABRINA; PEDRINI, NICOLÁS
Lugar:
Pittsburgh, Pennsylvania
Reunión:
Congreso; 46th Annual Meeting of the Society for Invertebrate Pathology Conference on Invertebrate Pathology and Microbial Control; 2013
Institución organizadora:
Society for Invertebrate Pathology
Resumen:
The chemical control of the Chagas disease vector Triatoma infestans is endangered by the emergence of pyrethroid resistance. Among alternative control tools, laboratory and field assays showed the efficacy of the entomopathogenic fungus Beauveria bassiana strain GHA to kill T. infestans. Several fungal isolates were shown to tolerate well deltamethrin doses similar to those used for T. infestans control, providing evidence that the combination of deltamethrin with entomopathogenic fungi might be used in future control strategies. In this work, the effect of deltamethrin on fungal growth, gene expression and enzyme activity in relation to detoxification, antioxidant response and oxidative stress levels was studied to evaluate fungal tolerance to deltamethrin. The mean inhibitory concentration (IC50) was 50 μg deltamethrin/cm2. Cytochrome P450genes were differentially expressed; cyp52X1 and cyp617N1 transcripts were > 2-fold induced, followed by cyp655C1 (1.8-fold). Minor effects were observed on genes encoding for other P450s, epoxide hydrolase and glutathione S-transferase (gst). Superoxide dismutase (sod) genes showed induction levels ≤ 2-fold, catalase (cat) and glutathione peroxidase (gpx) genes were also induced ~ 2-3-fold and < 2-fold, respectively. The activities of enzymes participating in the antioxidant defense system and phase II detoxification were also evaluated; SOD, CAT and GST activity showed significant differences with deltamethrin concentration. Lipid peroxidation levels and free proline content were also altered. We conclude that B. bassiana strain GHA can be used combined with deltamethrin without significant metabolic detrimental effects. This combination will help optimizing the benefits and increasing the efficacy of vector control tools.