INVESTIGADORES
LAVARIAS Sabrina Maria Luisa
artículos
Título:
CHANGES IN PHOSPHATIDYLCHOLINE MOLECULAR SPECIES IN THE SHRIMP MACROBRACHIUM BORELLII IN RESPONSE TO WATER-SOLUBLE FRACTION OF PETROLEUM.
Autor/es:
LAVARIAS, SABRINA MARIA LUISA; DREON, MARCOS S.; POLLERO, RICARDO J.; HERAS, HORACIO
Revista:
LIPIDS
Editorial:
AOCS Press
Referencias:
Año: 2005 vol. 40 p. 487 - 494
ISSN:
0024-4201
Resumen:
The effect of the water-soluble fraction (WSF) of crude oil on the lipid content, lipid classes, fatty acids and phosphatidylcholine (PC) molecular species was studied in high-phospholipid (hepatopancreas, HP) and low-phospholipid (eggs) models of a freshwater crustacean. After a 21-day exposure to sublethal concentration of WSF, a significant decrease in shrimp total lipids was observed, though no alterations could be detected in HP or egg lipid content. Triacylglicerol/phospholipid ratios increased in HP and decreased in eggs, suggesting alterations either in the mobilization of triacylglycerols to phospholipid pools or in the energy balance. The fatty acid (FA) composition of PG (phosphoglicerides) in HP and eggs was dominated by polyunsaturated FA (PUFA), while the n-3/n-6 ratio was not affected by WSF exposure, though there was a significant increase in HP 18:1n-9. Analysis of PC molecular species by HPLC-ELSD showed the presence of 15 species. 16:0/18:1, 18:1/18:2, 16:0/20:5 and 16:1/20:5 being the major species of HP. PC molecular species in eggs showed a different pattern, dominated by 16:0/18:1 and 18:1/18:2. 10 PC molecular species contained 22:6n-3, 20:5n-3 and 20:4 n-6. Small amounts of di-PUFA species were also found. WSF exposure altered PC molecular species in both tissues. The 4 major HP molecular species and most PUFA-containing ones decreased. This was compensated by an increase in 16:1/18:1 (152 %) and 18:1/18:1 (50 %). The major egg PC molecular species decreased, while PUFA-containing ones increased their proportion. The contrasting response of both tissues to WSF contamination suggests the presence of different homeostatic mechanisms.