Sensitivity of histopathological and histochemical parameters in the digestive gland of the apple snail Pomacea canaliculata exposed to cypermethrin

ARRIGHETTI, FLORENCIA; LANDRO, SONIA M.; LAVARÍAS, SABRINA M.L.

AQUATIC TOXICOLOGY

ELSEVIER SCIENCE BV

Año: 2022 vol. 252

0166-445X

The aim of this study was to evaluate the toxic effects of the pesticide cypermethrin(CYP) in the digestive gland of the apple snail Pomacea canaliculata analysinghistological and histochemical alterations. Adult snails were exposed to sublethal CYPconcentrations (10, 25, and 100 μg/L ) under acute (1 day) and sub-chronicconditions (14 days). Histological analyses of the morphology of the digestive glandwere performed and a histopathological condition index (HI) was calculated. Also, bothintracellular accumulation of lipofuscins (LF) and neutral lipids (NL) were evaluated.CYP exposure induced tissue damage on this organ, such as disorganisation of theconnective tissue, fibrosis, haemocyte infiltration, atrophy, and necrosis under acuteand sub-chronic conditions. These alterations, integrated into a single HI value,revealed notable CYP effects during both acute and sub-chronic exposures. Cell typereplacement, measured as Vv BAS , was only observed in the sub-chronic treatment.Under acute conditions, the pyrethroid affected NL accumulation at the highestconcentration, while at sub-chronic conditions NL accumulation was only observed atthe lowest concentrations. P. canaliculata also showed a dose-dependent responseto LF under acute CYP exposure conditions. However, under sub-chronic conditions,this parameter was not sensitive to pesticide exposition. All these relevant structurallesions may affect the digestive gland normal function, even though the speciespresented additional mechanisms that help it to tolerate the exposure to pollutants.This study showed that some histological and histochemical parameters are sensitivein P. canaliculata at CYP concentrations to which the snail could be exposed in theenvironments it inhabits.