First phylogenetic analysis of the genus Alotanypus (Diptera: Chironomidae: Tanypodinae)

SIRI, AUGUSTO; DONATO, MARIANO

La Plata

Otro; IX Reunión Argentina de Cladística y Biogeografía; 2010

The Macropelopini genus Alotanypus is presently known byfour species,Avenustus (Coquillett) and Aaris Roback from the Nearctic Region, A dalyupensis (Freeman) from the Australian Region and A kuroberobustus (Sasa and Okazawa) from the Palearctic Region.ln the present analysis, we also included to A vittigera (Edwards) from Patagonia,Argentina.This species was firstly described as Anatopynia vittigera, and we transfered to Alotanypus (unpublished data). In addition, we included one representative species of each of Macropelopini tribe, two of Pentaneurini and one species of Natarsini. Characters used for the analysis consist of 56 continuous characters and 48 discrete ones. Characters of male and female adults, as well as characters of pupa and larva were included in the analysis.A data set with all quantitative characters standardized and expressed as ranges together with discrete characters coded as nonadditive were analyzed with the program TNT version 1.1 under maximum parsimony using equal and implied weights.Analyses with implied weighting were conducted by means of values for the concavÍty constant k= 5-16. AII tree searches were performed using a Wagner tree as startÍng tree and 1000 random addition sequen ces plus TBR with 100 trees to save per replication, followed by TBR branch swapping. The results obtained in this analysis were estÍmated as absolute and GC (Group presentlContradicted) frequencies with 1000 replicates of jackkinfe symmetrical resampling plus TBR (jackknife symmetric resampling ís not affected by weighted characters), 10 random addition sequences, and saving 100 trees per replicate. Results of the analysis show Guassutanypus oliveirai as the sister group the genus Alotanypus.The clade including all Alotanypus species has a high support, sharing the "antennal ratio", "Ieg ratio 111", "wing length", "absence of posnotals", "presence of tibial comb 1" and "spatullated or pointed claws" on male adults;"LlW ofthoracic horn", "relative possition oflateral seta I on abdominal s.VII andVIII" of pupa; "cephalic index" on larva.Alotanypus vittigera is closely related with Akuroberobustus, and the clade is highly supported. The clade [A aris-A vittigera-A kuroberobustus] as well as the clade [A dalyupensis-A aris-A vittigera-A kuroberobustus] have a low support.