INVESTIGADORES
FOSSATI Carlos Alberto
congresos y reuniones científicas
Título:
(55) ORAL IMMUNOTHERAPY FOR FOOD ALLERGY IN A COW`S MILK ALLERGY MOUSE MODEL
Autor/es:
ORSINI DELGADO L; SMALDINI P; FOSSATI CA; DOCENA GH,
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Food allergy is an immunological
disorder caused by a breakdown in immunologic tolerance. Treatment consists of
strict avoidance of food allergens. However, corrective therapies are being
studied. We aimed to induce oral tolerance to cow`s milk proteins (CMP) using
CMP in an IgE-mediated food allergy mouse model.
Balb/c mice were intragastrically
given CMP prior to (CMPprev)or after
(CMPdes)sensitization with CMP plus
cholera toxin. Mice were orally challenged with CMP and the immune response was
evaluated with in vitro (serum IgE, IgG1, IgG2a; TNF-α, IL-6,
IL-17A, IL-5, IL-13, IFN-γ, IL-10 and TGF-β expression
?qPCR- andproduction ?ELISA or flow citometry-by spleen cells and intestinal
mucosa) and in vivo assays (clinical score and cutaneous
tests).Histology was analyzed in gut specimens to evaluate the inflammatory
infiltrate.
We found a lower mean clinical
score in CMPprev and CMPdes mice after the oral challenge with CMP, as
compared to sensitized mice, with a negativization of skin tests in tolerized
mice. Immunological changes included decreased serum specific IgE (1,57±0,2 vs
0,87±0.2 CMPdes;0,52±0,1 CMPprev) and IgG1(2,07±0,7 vs 0,91±0,2 CMPdes; 0,11±0,05 CMPprev), decreased protein and mRNA level of
IL-6, IL-5 and IL-13 (p<0,05), and induction of regulatory Tcells in
duodenum of treated mice (0,73±0,01% vs 1,055±0,01% CMPdes; and vs 11,10±1,6% CMPprev) and in mesenteric lymph nodes
(0,373±0,3% vs 2,675±1,1% CMPprev) in
tolerized mice. TGF-β and IL-10 were induced in lamina propria
and we found increased intestinal CD4+ CD25+ FoxP3+ cells as source of TGF-β and IL-10.
Besides, a mild inflammatory infiltrate of mononuclear cells was observed in
duodenum of sensitized mice, but not in treated mice.
Oral
immunotherapy with milk proteins down-modulated the allergic specific immune
response in CMP-sensitized mice.This approach could provide a new therapeutic
anti-inflammatory intervention for CMP food allergy, either for prevention or
desensitization