INVESTIGADORES
FOSSATI Carlos Alberto
congresos y reuniones científicas
Título:
106. Oral Immunotherapy for Cow´s Milk Allergy Using a Mouse Model of Food Allergy,
Autor/es:
DOCENA, GH; SMALDINI P; ORSINI DELGADO L; CURCIARELLO R,; CANDREVA ANGELA; FOSSATI CA; PETRUCCELLI S,
Lugar:
Dublin
Reunión:
Congreso; Congreso del Grupo Europeo de Imunidad de Mucosas; 2012
Institución organizadora:
International Society for Mucosal Immunology
Resumen:
Food
allergy is caused by a breakdown in immunologic tolerance and there is no
approved therapy for this pathology. We aimed to induce oral tolerance to cow`s
milk proteins (CMP) using CMP and cross-reactive soy proteins (SP) in a food
allergy mouse model.
Balb/c
mice were gavaged with CMP (CMP Tol) or SP (PS Tol) prior to oral sensitization
with CMP and cholera toxin, and then orally challenged with CMP. Immune
response was evaluated with in vitro (serum IgE, IgG1, IgG2a and IgA
level; IL-5, IL-13 and IFN-γ secretion by splenocytes and Foxp3 T cells in the
intestinal mucosa) and in vivo assays (clinical score and cutaneous
tests).
We
found a lower medium clinical score in tolerized mice with CMP or PS after the
oral challenge, as compared to sensitized mice, with a reduction in mast cell
reactivity in tolerized mice. Immunological changes include decreased specific
IgE (1,57±0,2 vs 1,06±0.3 SP Tol; 0,52±0,1 CMP Tol) and IgG1, decreased IL-5
and IL-13 at the protein and mRNA level and induction of regulatory Tcells in
lamina propria, Peyer´s patchs and mesenteric lymph nodes in tolerized mice.
IgG2a and IgA levels were not modified.
In conclusion, oral immunotherapy based on
cross-reactivity between CMP and SP can be used to down-modulate the allergic
specific immune response in CMP-sensitized mice. This approach could provide a
new therapeutic intervention for CMP food allergy