Antibody reacftivity to OMP31 from Brucella melitensis in human and animal infections by smooth and rough Bruecellae

CASSATARO J,; PASKEVICH K,; BURNO L,; WALLACH JC,; CARLOS ALBERTO FOSSATI; BALDI PC,

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY.

ASM

Año: 2004 vol. 11 p. 111 - 114

1071-412X

Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, exceptBrucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 (B. melitensis) and tested its recognition by sera from humans and animals suffering from brucellosis by an indirect enzyme-linked immunosorbent assay (ELISA). Serum samples from 74 patients, 57 sheep, and 47 dogs were analyzed; brucellosis was confirmed by bacteriological isolation in all ovine and canine cases and 31 human cases of brucellosis. Thirty-five patients (47%) were positive for antibodies to Omp31, including seven cases of Brucella suis infection, two cases of B. abortus infection, and three cases of B. melitensis infection.