STUDY OF THE JOINT ACTION OF STEROID HORMONES RECEPTORS IN THE MECHANISMS THAT CONTROL INFLAMMATORY RESPONSE

NOTO GUILLEN, M; PECCI, A; OGARA, MF

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Inflammation is a vital defense mechanism against infections or damaged tissue. While this response is active, homeostatic processes that balance pro- and anti-inflammatory signaling pathways are induced. If unbalanced, these control mechanisms lead to deregulated inflammation. Studies about inflammatory response allowed the development of therapies against several disorders. Glucocorticoids have been widely used as immunosupressors and anti-inflammatory drugs. However, other steroid ligands and their receptors (e.g. LXRs) have been proved to act as modulators of the inflammatory response. Steroid receptors share structural and functional properties. Cross action between some of them was found in several tissues and cellular response. Nevertheless, there are currently no studies that evaluate whether GR and LXRs interact when activated in immune cells. In this work, we use as model the pro-monocytic human myeloid leukaemia cell line U937, which can be differentiated into macrophage-like cells in the presence of phorbol myristate acetate (PMA). Treatment with PMA stimulates the expression of several pro-inflammatory genes such as CD11b, IL6, IL8, TNF-α and COX2 (p