Antimutagenic activity of a protein isolated from C. betacea mature fruit.

ORDOÑEZ R., SAYAGO J., NIEVA MORENO M.I., ISLA M.I

Pinamar. Argentina

Congreso; - 41th Annual meeting. XLI reunión Anual Argentine Society for Biochemistry and Molecular Biology Research. Molecular. PABMB X10 th Congreso Panamerican Association for Biochemistry and Molecular Biology.; 2005

PAMB, SAIB, SAN

Cyphomandra betacea (Cav.) Sendt is a subtropical fruit native from Andean region of Perú, Argentine and Chile. It has been used like food in salad and jellie preparations. Nevertheless, their functional properties have not been exploited yet. In a previous work, we have isolated and purified an invertase inhibitory protein (IIP) from mature fruit. This protein exerts in vitro inhibitory action on hydrolases isolated from phytopathogenic bacteria and fungi. It also can inhibit the growth of them. Recently, we demonstrated their antioxidant capacity, reactive oxygen species scavenging activity (O2·- and HO·) and DPPH-radical scavenging activity.  In this work, we evaluated the toxic, mutagenic and antimutagenic effects of IIP by using a short-term test. The assay using Salmonella typhimurium TA 98 and TA 100 was performed in presence of two mutagens, isoquinoline (IQ) and 4-nitro o-phenylenediamine (NPD), with and without metabolic activation. IIP showed no toxicity to assayed strains at concentrations that have antimicrobial and antioxidant activities (5-250 µg/ml). The IIP showed MR (mutagenicity relation) values below 2, which indicate that the protein does not exert effect mutagenic. IIP was able to inhibit the NPD-mutagenicity. According with our results, we could suggest the potential application of this protein in the agriculture control of phytopathogenic organism development in post-harvested fruit and vegetable.