CHARACTERIZATION OF ANTIBIOTIC PHARMACEUTICAL FORMULATION CONTAINING BACCHARIS EXTRACTS

NUÑO G; ZAMPINI IC, ALBERTO MR, ORDOÑEZ RM; ARIAS M, ISLA MI

SM Tucumán

Congreso; VII Congreso Argentina de Microbiología General; 2011

Sociedad Argentina de Microbiologia

Baccharis incarum (Wedd.) Perkins (syn.: Baccharis tola Phil.) known under the popular names of lejia, tola and baila buena is common in the arid Andean at 3,800 meters above sea level in Antofagasta de la Sierra, Catamarca, Argentina. Previous phytochemical studies on the leaves and top parts (aerial parts) of B. incarum described the isolation of chlorogenic acid and flavones. The anti-inflammatory, antioxidant and anti-xanthine oxidase effects and the antibacterial activity on antibiotic resistant Gram positive and Gram negative bacteria were demonstrated in B. incarum ethanolic extract. The aim of the present work was to obtain an antibiotic pharmaceutical formulation containing B. incarum ethanolic extract. The tincture was incorporated in a topical pharmaceutical formulation (hydrogel). The hydrogel containing Baccharis extract showed microbiological, physical and functional stability during storage for 90 days. The preparation was active against antibiotic-multi-resistant Gram negative and Gram positive bacteria and was not genotoxic in the Salmonella typhimurium assays. Studies which measure the drug release as determination of bioavailability were also carried out using the Franz diffusion cell. By HPLC analysis, two compounds (chlorogenic acid and 4’, 5-dihydroxy-3’, 3, 6, 7, 8-pentamethoxyflavone) were identified in the tincture and in the receptor solution of the Franz diffusion cell. The flavone also showed antimicrobial activity. The results obtained demonstrated that some natural compounds with antibacterial activities from B. incarum are released from the pharmaceutical formulations and this preparation could be used as topic antibiotic.