INVESTIGADORES
GARRIZ andres
congresos y reuniones científicas
Título:
PUTRESCINE PARTICIPATES IN PLANT DEFENSE IN ARABIDOPSIS THALIANA BY REGULATING CELL DEATH AND THE OXIDATIVE BURST
Autor/es:
ANDRÉS GÁRRIZ; BACH-PAGES M; SOLMI LEANDRO; OA RUIZ; FM ROMERO; PRESTON G; FR ROSSI
Lugar:
Salta
Reunión:
Congreso; Joint LV Annual SAIB Meeting and XIV PABMB Congress; 2019
Resumen:
Polyamines are a group of compounds with multiple physiological roles in all organisms. In plants, the metabolism of polyamines is altered uponpathogen invasion, which generally leads to an increment in its concentration and results in the activation of different defence mechanisms. Inthis trend, we previously showed that infection of Arabidopsis with virulent Pseudomonas syringae (Pst) provokes a marked increase in theconcentration of the polyamine putrescine (Put) in the apoplastic space, which is explained mainly by the upregulation of its biosynthesis and theremobilization from the intracellular compartments. In the present work, we assessed the functions played by this polyamine in plant immunity.Interestingly, a mutant plant line with reduced levels of Put showed increased cell death upon infection and showed a higher susceptibility tovirulent and non-virulent strains of Pst compared to wild-type plants, suggesting that the accumulation of Put is required for plant tolerance.Interestingly, a deeper exploration of the mechanistic role played by Put demonstrated that this polyamine inhibits the calcium influx and theoxidative burst mediated by NADPH oxidases that is induced during plant defence activation. On the other hand, we also demonstrated thatArabidopsis plants infected with non-virulent strains of Pst show higher Put accumulation compared to those infected by virulent strains, whichis directly correlated with the induction of plant cell death. These results suggest that a fine-tuned spatio-temporal regulation of the metabolismof Put modulates different processes associated to the defence against pathogens in plants