ARGINYLTRANSFERASE (Ate1) REGULATES STRESS RESPONSE TO BORTEZOMIB OF HUMAN GLIOMA CELLS.

BONNET, LAURA V.; JÉSICA FLORES MARTÍN; PALANDRI ANABELA; HALLAK, MARTA E.; GALIANO, MAURICIO R.

Paraná, Entre Ríos

Congreso; Congreso SAIB 2018; 2018

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The enzyme Ate1 mediates the post-translational addition of an Arg to proteins bearing acidic N-terminal amino acids that are mainly target toproteasomal degradation or macroautophagy. Previous studies reported that Ate1 downregulation suppresses cell death induced by differentstressors. In addition, Ate1-knockout fibroblasts exhibit tumorigenic properties, including exacerbated contact-independent growth andchromosomal aberrations. We recently reported that increased cell membrane exposure of an Ate1 substrate (arginylated calreticulin) modulatesthe sensitivity to proteasomal inhibitor bortezomib (BT) of human oligodendroglioma (HOG) cells. Hence, further assessment is required todetermine the Ate1 implication in the tumorigenic progression of BT treated cells. HOG cell death induced by BT comes through a mechanismthat involves activation of the unfolded protein response (UPR) mediators, including the transcriptional activation of the spliced mRNA xbp1 and concomitant upregulation of DR5-R membrane expression. Moreover, we do not observed changes in autophagy flux at different time and drugdoses. Strikingly, we found that Ate1 knockdown in HOG increases their sensitivity to BT in a macroautophagy independent way, suggestingthat apoptosis of glioma cells induced by BT is strongly influenced by Ate1 expression. We postulates that Ate1 is an essential enzyme thatregulates stress response and cell fate controlling the tumorigenic progress of cancer cells.