Protein coating defines the biological identity of nanoparticles: relevance of protein-protein interactions

CECILIA VASTI; LAURA V. BONNET; MAURICIO R. GALIANO; RICARDO ROJAS; CARLA E. GIACOMELLI

Buenos Aires

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017

In the area of biomedicine, the use of nanoparticles (NPs) as carriers of therapeutic or theranostic agents has increased in the last years. The interaction with proteins present in the biological media leads to the formation of the so-called ?protein corona?, which defines the biological identity of NPs.  This work explore the relation between the synthetic identity of layered double hydroxides nanoparticles (LDH-NPs), their biological identity in cell culture media and the effect of the latter on the cellular response. With such a purpose, the synthetic identity of LDH-NPs was modified by coating with either a single protein (albumin) or a complex mixture of them (fetal bovine serum). Then, the prepared NPs were dispersed in cell culture medium to study the effect in the protein corona formation. A proteomic approach was used to identify the protein corona molecules by LC-MS, the protein-protein interactions (PPI) network was constructed with a novel bioinformatic tool and the cellular response was evaluated via flow cytometry.