INVESTIGADORES
GALIANO Mauricio Raul
congresos y reuniones científicas
Título:
Protein coating defines the biological identity of nanoparticles: relevance of protein-protein interactions.
Autor/es:
CECILIA VASTI; LAURA V. BONNET; MAURICIO R. GALIANO; RICARDO ROJAS; CARLA E. GIACOMELLI
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión conjunta de Sociedades de Biociencias; 2017
Resumen:
In the area of biomedicine, the use of nanoparticles (NPs) as carriers of therapeutic or theranostic agents has increased in the last years. The interaction with proteins present in the biological media leads to the formation of the so-called ?protein corona?, which defines the biological identity of NPs. This work explore the relation between the synthetic identity of layered double hydroxides nanoparticles (LDH-NPs), their biological identity in cell culture media and the effect of the latter on the cellular response. With such a purpose, the synthetic identity of LDH-NPs was modified by coating with either a single protein (albumin) or a complex mixture of them (fetal bovine serum). Then, the prepared NPs were dispersed in cell culture medium to study the effect in the protein corona formation. A proteomic approach was used to identify the protein corona molecules by LC-MS, the protein-protein interactions (PPI) network was constructed with a novel bioinformatic tool and the cellular response was evaluated via flow cytometry. We have successively applied two strategies based on adsorbing single albumin (LDH@ALB) or a complex protein mixture from fetal bovine serum (LDH@FBS) to keep the nanometer size in cell culture medium. These new synthetic identities define their biological identities in such a way that the composition of the protein corona as well as the protein-protein interactions are changed. Furthermore, electrostatic interactions alone appear not to be the only driving force regulating the interactions between NPs, proteins and cells but specific biological recognition also play a fundamental role. However, the biological identities do not affect the interactions with cells as both LDH@ALB and LDH@FBS are highly internalized without generating any cytotoxicity, demonstrating their safety as potential drug carriers.