INVESTIGADORES
GLEISER gabriela Laura
artículos
Título:
Isolation and characterization of microsatellite loci in Acer opalus (Aceraceae), a sexually polymorphic tree, through an enriched genomic library
Autor/es:
SEGARRA-MORAGUES, J. G.; GLEISER, G.; GONZÁLEZ-CANDELAS, F.
Revista:
CONSERVATION GENETICS
Editorial:
SPRINGER
Referencias:
Año: 2008 vol. 9 p. 1059 - 1062
ISSN:
1566-0621
Resumen:
An enrichment protocol was used to isolate andcharacterize microsatellite loci in Acer opalus, a Mediterraneantree species. Highly polymorphic microsatellite lociwere required for paternity analyses in a population of thisspecies. Eight microsatellite loci were amplified and a totalof 87 alleles were detected in a sample of 142 individualsfrom one population, allowing the identification of eachindividual with a unique multilocus genotype. The paternityexclusion probabilities varied from 0.261 for locusAop820 to 0.806 for locus Aop450, and the parent-pairexclusion probabilities varied from 0.433 for Aop820 to0.940 for Aop450. The cumulative probabilities of exclusionfor paternity and parentage of the eight loci were bothhigher than 0.999, supporting the usefulness of thesemicrosatellite loci for future paternity and parentage analysesin A. opalus. Cross-species transferability was alsoassayed, supporting their potential use in other eight Acerspecies.
