Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries

SEGARRA-MORAGUES, J. G.; GLEISER, G.

CONSERVATION GENETICS

SPRINGER

Año: 2009 vol. 10 p. 571 - 575

1566-0621

An enrichment protocol was used to isolate andcharacterise microsatellite loci in Rosmarinus officinalis, aMediterranean chamephyte. Twelve microsatellite loci werecharacterised and amplified a total of 117 alleles in a sampleof 30 individuals from one population, with an average of9.75 alleles per locus. Observed heterozygosities rangedfrom 0.333 to 0.900. Cross-species transferability was alsoassayed in the two other species of the genus. The cumulatedprobabilities of exclusion for paternity and parentage of the12 loci were of 0.999971 and 1, respectively, supporting theusefulness of these microsatellite loci for parentage analyses.Nine out of 12 microsatellite loci amplified in the twospecies and were polymorphic detecting a total of 49 and 45in R. eriocalyx and R. tomentosus, respectively. Twentytwoalleles were exclusive of R. eriocalyx and 12 of R. tomentosus,additionally, three alleles were shared betweenthese two species but were otherwise absent in the analysedindividuals of R. officinalis. In total, this set of markersamplified 154 different microsatellite alleles, supportingtheir usefulness to conduct population genetic, reproductivebiology and hybridisation studies in Rosmarinus.