Efficient labeling of dendritic cells with a clinical applicable perfluorpolyether compound for quantitative 19F MRI tracking in cancer patients,

F. BONETTO; M. SRINIVAS; R. MAILLIARD; E. T. AHRENS; A. HEERSCHAP; C. G. FIGDOR; J. DE VRIES

Honolulu, Hawai, Estados Unidos

Conferencia; 17th Conference of the International Society for Magnetic Resonance in Medicine; 2009

International Society for Magnetic Resonance in Medicine (ISMRM)

Tracking of cells using contrast agents with magnetic resonance imaging provides detailed anatomical information but it lacks quantitative information. A new perfluoropolyether nanoemulsion was studied for labeling of dendritic cells for 19F MRI. Our results demonstrate that the compound is taken up by the cells and does not hamper cell function ( viability, maturation and mRNA uptake).Embedded dendritic cells in gelatin can be easily imaged and the sensitivity was determined to be 500 cells/mm3 at 7T. Our results suggest that the compound is suitable for cell tracking in vivo by 1H/19F MRI providing both anatomical and quantitative information.