A novel 19F MRI-based migration assay applied to tracking primary human dendritic cells.

F. BONETTO; M. SRINIVAS; B. WEIGELIN; L. J. CRUZ; A. HEERSCHAP; C. G. FIGDOR; J. DE VRIES

Varsovia

Encuentro; 5th European Molecular Imaging Meeting -; 2010

In the present study we showed that 19F-CSI and 19F-MRI can be used to track andquantify cell migration in 3D collagen scaffold assays. The number of cells in the initial cell layerwas in the order of the typical cell bolus injection in patients [1] and the migration rates weremeasured to be in the order of 2%, similar to the obtained in clinical results. The study showed thatthe initial cell density plays a decisive role in DCs migration. Such information could be used tooptimize cell migration in a clinical setting. Thus, this assay could be suitably simulating in vivoclinical conditions, giving the potential chance to use it to analyze DCs migratory problems usingMRI.