Comparison of Direct Fluorescent Antibody Test and bacteriological culture for detection of Brucella spp. Application to demonstrate B. suis in swine tissues

ESTEIN S.M., BENCE A.R., CACCIATO C.S., ECHEVARRÍA H.M., LUCCHESI E., SOTO P

Revista Veterinaria

FCV, UNNE

Lugar: Corrientes; Año: 2019

1668-4834

Methods available for detection of Brucella spp. from different specimens include bacteriological culture or detection of specific DNA fragments by Polymerase Chain Reaction. The use of fluorescein-labeled anti-Brucella globulin for demonstrating this antigen in animal tissues is a simple, easy, reproducible, cheap and fast technique. The aim of this work was evaluate the gammaglobulin fraction of polyclonal anti-Brucella abortus serum labeled with fluorescein (FITC-labeled antibody) 1) against different smooth and rough Brucella spp., 2) against bacterium of other genus and 3) to compare Direct Fluorescence Antibody Test results with bacteriological culture for the detection of B. suis in different tissues from infected animals. This conjugate stained all Brucella spp. with different intensities but it did not stain any heterologous bacterium tested. Background fluorescence associated with its use on smears from infected sources of different specimens was particularly low. The most of infected tissues showed the presence of yellowish-green fluorescent organisms with brucella morphology. The tested FITC-labeled antibody allows a quick, effective and inexpensive diagnosis of brucellosis.