INVESTIGADORES
ESTEIN Silvia Marcela
artículos
Título:
Brucella Lumazine Synthase Elicited a Th1/Th2 Mixed Immune Response and Protected Mice Against Brucella abortus 544 Challenge Independently of the Adjuvant Formulation Used
Autor/es:
VELIKOVSKY C.A., GOLDBAUM F.A., CASSATARO J., ESTEIN S. M., BOWDEN R. A., BRUNO L., FOSSATI C.A., GIAMBARTOLOMEI G.H
Revista:
INFECTION AND IMMUNITY
Editorial:
American Society of Microbiology
Referencias:
Año: 2003 vol. 71 p. 5750 - 5755
ISSN:
0019-9567
Resumen:
The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS)
administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence
or the presence of aluminum hydroxide gel (BLS-Al), monophosphoryl lipid A (BLS-MPA), or incomplete
Freunds adjuvant (BLS-IFA). rBLS per se induced a vigorous immunoglobulin G (IgG) response, with high
titers of IgG1 as well as IgG2. All the adjuvants increased this response; the BLS-IFA formulation was the most
effective at inducing BLS-specific IgG antibodies. In addition, after in vitro stimulation with rBLS, spleen cells
from BLS-IFA-, BLS-Al-, or BLS-MPA-immunized mice proliferated and produced interleukin-2 (IL-2), gamma
interferon (IFN-_), IL-10, and IL-4, suggesting the induction of a mixed Th1-Th2 response. Immunization with
rBLS protected mice against challenge with B. abortus 544. The levels of protection in the spleen were similar
for all adjuvants, but only BLS-Al and BLS-IFA were effective in the liver. Our results indicate that BLS might
be a useful candidate for the development of subunit vaccines against brucellosis, since it elicits antigen-specific cellular responses, with production of IFN-_ and protection, independently of the adjuvant formulation used.