INVESTIGADORES
ESTEIN Silvia Marcela
artículos
Título:
A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB/c mice
Autor/es:
VELIKOVSKY C.A., CASSATARO J., GIAMBARTOLOMEI G.H., GOLDBAUM F.A., ESTEIN S.M., BOWDEN R.A., BRUNO L., FOSSATI C.A., SPITZ M
Revista:
INFECTION AND IMMUNITY
Editorial:
American Society of Microbiology
Referencias:
Año: 2002 vol. 70 p. 2507 - 2511
ISSN:
0019-9567
Resumen:
This study was conducted to evaluate the immunogenicity of the Brucella abortus lumazine synthase (BLS)
gene cloned into the pcDNA3 plasmid, which is driven by the cytomegalovirus promoter. Injection of plasmid
DNA carrying the BLS gene (pcDNA-BLS) into BALB/c mice elicited both humoral and cellular immune
responses. Antibodies to the encoded BLS included immunoglobulin G1 (IgG1) IgG2a, IgG2b, IgG3, and IgM
isotypes. Animals injected with pcDNA-BLS exhibited a dominance of IgG2a over IgG1. In addition, spleen
cells from vaccinated animals produced interleukin-2 and gamma interferon but not IL-10 or IL-4 after in vitro
stimulation with recombinant BLS (rBLS), suggesting the induction of a Th1 response. Protection was
evaluated by comparing the levels of infection in the spleens of vaccinated mice challenged with B. abortus 544.
Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice
immunized with rBLS produced a significant humoral response but did not show a specific cellular response
or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the
production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of
vaccination against brucellosis.
Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice
immunized with rBLS produced a significant humoral response but did not show a specific cellular response
or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the
production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of
vaccination against brucellosis.
gene cloned into the pcDNA3 plasmid, which is driven by the cytomegalovirus promoter. Injection of plasmid
DNA carrying the BLS gene (pcDNA-BLS) into BALB/c mice elicited both humoral and cellular immune
responses. Antibodies to the encoded BLS included immunoglobulin G1 (IgG1) IgG2a, IgG2b, IgG3, and IgM
isotypes. Animals injected with pcDNA-BLS exhibited a dominance of IgG2a over IgG1. In addition, spleen
cells from vaccinated animals produced interleukin-2 and gamma interferon but not IL-10 or IL-4 after in vitro
stimulation with recombinant BLS (rBLS), suggesting the induction of a Th1 response. Protection was
evaluated by comparing the levels of infection in the spleens of vaccinated mice challenged with B. abortus 544.
Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice
immunized with rBLS produced a significant humoral response but did not show a specific cellular response
or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the
production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of
vaccination against brucellosis.
Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice
immunized with rBLS produced a significant humoral response but did not show a specific cellular response
or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the
production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of
vaccination against brucellosis.