INVESTIGADORES
ESTEIN Silvia Marcela
artículos
Título:
The protein moiety of Brucella abortus outer membrane protein Omp16 is a new bacterial pathogen associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response and is a promising self-adjuvanting vaccine against systemic
Autor/es:
K A. PASQUEVICH, C. GARCÍA SAMARTINO, L. M. CORIA, S. M. ESTEIN, A. ZWERDLING, A., E. IBAÑEZ, P. BARRIONUEVO, F. SOUZA DE OLIVEIRA, J. BORKOWSKI¶, S. COSTA OLIVEIRA, H. WARZECHA¶, G.H. GIAMBARTOLOMEI, J. CASSATARO.
Revista:
JOURNAL OF IMMUNOLOGY
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Año: 2010 vol. 184 p. 5200 - 5212
ISSN:
0022-1767
Resumen:
Knowing the inherent stimulatory properties of the lipid moiety of bacterial lipoproteins, we first hypothesized that Brucella abortusouter membrane protein (Omp)16 lipoprotein would be able to elicit a protective immune response without the need of externaladjuvants. In this study, we demonstrate that Omp16 administered by the i.p. route confers significant protection against B. abortusinfection and that the protective response evoked is independent of the protein lipidation. To date, Omp16 is the first Brucella proteinthat without the requirement of external adjuvants is able to induce similar protection levels to the control live vaccine S19. Moreover,the protein portion of Omp16 (unlipidated Omp16 [U-Omp16]) elicits a protective response when administered by the oral route.Either systemic or oral immunization with U-Omp16 elicits a Th1-specific response. These abilities of U-Omp16 indicate that it isendowed with self-adjuvanting properties. The adjuvanticity of U-Omp16 could be explained, at least in part, by its capacity toactivate dendritic cells in vivo. U-Omp16 is also able to stimulate dendritic cells and macrophages in vitro. The latter property and itsability to induce a protective Th1 immune response against B. abortus infection have been found to be TLR4 dependent. The facts thatU-Omp16 is an oral protectiveAgand possesses a mucosal self-adjuvanting property led us to develop a plant-made vaccine expressingU-Omp16. Our results indicate that plant-expressed recombinant U-Omp16 is able to confer protective immunity, when given orally,indicating that a plant-based oral vaccine expressing U-Omp16 could be a valuable approach to controlling this disease