INVESTIGADORES
ESPELT Maria Victoria
artículos
Título:
Hormone uptake and morphological changes in cultured pituicytes exposed to oxytocin and vasopressin.
Autor/es:
ESPELT MV, BILINSKI M, TRAMEZZANI JH.
Revista:
BIOCELL
Referencias:
Año: 1998 p. 103 - 108
ISSN:
0327-9545
Resumen:
Enzymatically dispersed cells, isolated from adult female rat neural
lobes, were cultured for 7 days. Routine cultures showed pituicytes
with compact, sometimes ovoid, cell bodies. The cytoplasmic processes
of these cells exhibited several varicosities and made contact with
neighboring cells forming networks. The cultured pituicytes were
immunocytochemically characterized using antisera to glial fibrillary
acidic protein and to S-100. Most pituicytes, when exposed during
culture to oxytocin (OXY) and vasopressin (VP; 1 microM each), were
devoid of their characteristic processes. Immunocytochemical staining
for OXY or VP revealed that the pituicytes were capable of
incorporating these hormones during culture. In cultures without added
hormones, no significant staining reaction for OXY or VP could be
detected. The lack of projections in pituicytes exposed to the hormones
during culture is in agreement with the morphological changes observed
by other authors in situ after acute hormone release. The uptake of OXY
and VP may be indicative for a regulatory mechanism, by which the
pituicytes control the amount of hormones present in the intercellular
space.