ACHYROCLINE SATUREIOIDES PROTECTS FROM GENOTOXIC AND OXIDATIVE DAMAGE INDUCED BY ZEARALENONE IN BALB/C MICE

SABINI MARÍA CAROLA; CARIDDI LAURA NOELIA; MAÑAS FERNANDO; ESCOBAR FRANCO MATÍAS; SABINI LILIANA INÉS; DALCERO ANA MARIA

TAFÍ DEL VALLE

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

ASOCIACION DE BIOLOGIA DE TUCUMAN

ACHYROCLINE SATUREIOIDES PROTECTS FROM GENOTOXIC AND OXIDATIVE DAMAGE INDUCED BY ZEARALENONE IN BALB/C MICESabini MC1, Cariddi L1, Mañas F2, Escobar F1, Sabini L1, Dalcero A1.1Dpto Mic e Inm, FCsEFQyNat, UNRC. Ruta 36 Km 601. Río IV, Cba, Argentina. 2 Agro y Vet, UNRC. E-mail: csabini@exa.unrc.edu.ar.Mycotoxicoses occurs by ingestion of food contaminated with fungal toxins. Zearalenone (ZEA), produced by Fusarium species, induces toxic and genotoxic effects in humans and animals. A. satureioides has several medicinal properties such as antioxidant, antimicrobial, antiviral. The objective was to evaluate the ability of the cold aqueous extract (CAE) of A. satureioides to protect from genotoxic and oxidative damage induced by ZEA in mice. Obtention of CAE: plant material was extracted with distilled water at room temperature for 48 h and then the extractive liquid was lyophilized. Protection studies: groups of 4 Balb/C mice (20 g) were formed and were inoculated by intraperitoneal injection with different treatments: 1-CAE (50mg/kg body weight) + ZEA (40 mg/kg bw); 2-CAE (100 mg/kg) + ZEA (40 mg/kg); 3-Negative control: saline solution; 4- Positive control: cyclophosphamide 30 mg/kg; 5- ZEA Control: 40 mg/kg. At 24h post-injection mice were sacrificed and the antigenotoxicity was evaluated by micronucleus test in mouse bone marrow: according to Schmidt W. (1975) and, the antioxidant capacity of CAE was determined by thiobarbituric acid reactive substances (TBARS): according to Buege and Aust (1978), modified by Marcincak et al., (2003). Antigenotoxicity results indicated that the groups ZEA+ CAE at 50 and 100 mg/kg showed significant difference with ZEA control group (p